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LIU Xing-mei ZHANG Ying-ying WANG Yuan-yuan SHI Ming-jun XIAO Ying ZHANG Fan GUO Bing 《园艺学报》2019,35(12):2169-2174
AIM: To observe the changes of Notch1 expression and autophagy in the renal tissues of diabetic mice, and to explore the regulatory effect of Notch1 on tubulointerstitial fibrosis by inhibiting autophagy in diabetic nephro-pathy. METHODS: The mice were randomly divided into normal control group (db/m mice) and diabetes group (db/db mice), with 8 rats in each group. After 12 weeks of feeding, the mice were sacrificed and the corresponding biochemical indexes were measured. The protein expression of Notch1 in the renal tubular epithelial cells was observed by immunohistochemical staining. The protein levels of Notch1, PTEN, p-Akt (Thr308), Akt, p-mTOR (Ser2448), mTOR, LC3, P62, collagen type Ⅰ (Col-Ⅰ) and collagen type Ⅲ (Col-Ⅲ) were determined by Western blot. RESULTS: Compared with the db/m mice, the blood glucose, glycosylated hemoglobin, serum creatinine, triglyceride and total cholesterol were increased in the db/db mice (P<0.01). Renal tubular epithelial cell vacuolar degeneration, renal tubular expansion and interstitial inflammatory cell infiltration in db/db mouse renal tissues with HE staining were observed. The images of Masson staining showed collagenous fiber-like substance deposition in the glomerular capillaries and renal interstitium, and disarrangement of tubular structure in the renal tissues of db/db mice. The protein expression levels of PTEN and LC3-Ⅱ were decreased (P<0.01 or P<0.05), while the protein levels of Notch1, P62, p-mTOR (Ser2448), p-Akt (Thr308), Col-I and Col-III were increased in the db/db mice as compared with the db/m mice (P<0.01). However, no significant change of total mTOR and Akt proteins between the 2 groups was found. CONCLUSION: Notch1 protein expression was increased, PTEN expression was significantly reduced, Akt/mTOR pathway was activated, autophagy was inhibited, and fibrosis was aggravated in the renal tissues of the diabetic mice. 相似文献
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Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens 下载免费PDF全文
ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300
We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control. 相似文献
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AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP+-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP+ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP+ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP+-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis. 相似文献
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Sameer Kumar Chanda Venkata Ganga Rao Nadigatla Veera Prabha Rama Rachit K. Saxena Kulbhushan Saxena Hari D. Upadhyaya Moses Siambi Said N. Silim Kothapally Narasimha Reddy Anupama J. Hingane Mamta Sharma Shivali Sharma Stephen Dominic Lyimo Rose Ubwe Meshack Makenge Kananji Gad Paul Kiprotich Kimurto Manuel Amane Kennedy Kanenga Yuventino Obong Emanuel Monyo Chris Ojiewo Nagesh Kumar Mallela Venkata Jaganmohan Polineni Rao Prashanthi Lakkireddy Sudhakar Chourat Indraprakash Singh Sobhan Sajja Shruthi Hirikara Beliappa Rajeev K. Varshney 《Plant Breeding》2019,138(4):445-454
In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research. 相似文献
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作为拖拉机研发的重要验证手段,拖拉机室内台架试验可方便高效地为拖拉机模拟各种工况下的性能表现。首先探究动力总成控制技术、负载模拟技术、试验数据处理技术和试验结果评价技术4种拖拉机室内台架试验关键技术,结合试验技术的最新研究进展,对4种技术进行详细的阐述和分析。接着从动力总成控制算法、负载模拟算法、试验数据处理方法、试验结果评价指标体系4个方面对拖拉机室内台架试验技术进行讨论与展望。最后得出对动力总成算法进行优化、提高动态负载模拟精度、保持试验数据处理方法的前沿性、对试验结果综合客观评价是拖拉机室内台架试验技术的发展趋势,以期为我国拖拉机室内台架试验技术的发展提供参考。 相似文献
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分析了从南疆职业院校园艺技术专业农业应用化学教学中存在的问题,以提高该课程教学要求及园艺技术人才专业人才培养规格为目标,提出了对接教学领域与职业技能、职业素养,改革教学内容、课程体系、优化教学的方法和手段,为提高园艺技术专业服务育人本质,培养更多更好服务地方经济社会发展的高素质、高技能的园艺技术专业人才奠定重要的基础。 相似文献
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[目的/意义]目前中国畜牧业正处于加快转型的重要时期,了解相关技术的发展态势对于畜牧业的发展具有一定的借鉴意义。[方法/过程]本文以设施畜牧业领域技术研发的3个主要方向:笼舍技术、饲喂饮水技术和环境控制技术的相关专利为研究对象,利用专利计量的方法对其发展趋势、技术分支、申请人、各阶段重点专利等进行了分析。[结果/结论]笼舍、饲喂饮水和环境控制技术发展同步经历了萌芽期、波动发展期、快速发展期3个阶段,当前申请量呈现直线上升的态势;技术研发主体以企业为主,各研发单位之间技术布局差异性明显;近些年重点专利中的国内专利明显增多;笼舍技术方面装置研发均侧重于粪尿清除、垫板等技术;饲喂饮水技术方面饲喂技术的研究多于饮水技术,且均侧重于研发自动化设备;环境控制技术侧重于废弃物处理技术、空气调节技术和房舍清洁刷洗技术。 相似文献